Fig. 2
a PCR products of ITS-1 from promastigote DNA extract, 1. 100 bp ladder, 2. Negative control, 3. L. donovani, 4. L. aethiopica, 5. L. tropica, 6. L. major, 7. L. infantum, 8. L. chagasi, 9–12 clinical isolates. b PCR-ITS1-RFLP of the amplicon with Hha I, 1. 100 bp ladder, 2. L. donovani, 3. L. aethiopica, 4. L. tropica, 5. L. major, 6. L. infantum, 7. L. chagasi, 8–12 clinical isolate